Native Pig Neutrophil Products: Insights into Their Antimicrobial Activity

Cationic antimicrobial peptides are molecules with potential applications for treating infections due to their and immunomodulatory properties. The aim of this work was explore the activity mechanisms action a porcine neutrophil cathelicidin mixture (MPPN). Gram-positive Gram-negative bacteria were used determine minimum inhibitory concentration (MIC) experiments both time–kill kinetics effects on growth curves performed. Planar black lipid bilayer conductance measured analyze interaction MPPN bilayers. Visualization bacterial surfaces membrane alterations achieved using atomic force microscopy transmission electron microscopy. efflux pumps (EPs) studied an intracellular accumulation acridine orange (AO) assay. In E. coli, behaves as bactericide at high concentrations bacteriostatic lower concentrations. effect also observed slightly shorter periods in S. enterica. not active aureus. increase AO presence indicates that, least causes inhibition EP function. Observed detected variable events demonstrate strong Damage structure treated coli that induces surface. use AMPs capable inhibiting can be seen good tool combat resistance since they could alone or combination other conventional antibiotics which have become resistant.